合适的标准回肠可消化色氨酸/赖氨酸比率( SID Trp/Lys),对育肥后期母猪饲喂低蛋白日粮, 在改善生长性能，调控激素与肌肉氨基酸转运体的研究
W. F. Ma, S. H. Zhang, X. F.Zeng, X. T.Liu, C. Y. Xie, G. J. Zhang and S. Y. Qiao
本试验研究了不同标准回肠可消化色氨酸/赖氨酸比率 ( SID Trp/Lys)对饲喂低蛋白日粮（9.6%）并补充合成氨基酸在育肥母猪生长性能和胴体指数的影响。选用90头育肥母猪（89.1±5.1kg）进行35天的试验。玉米-麦麸基础日粮中添加合成色氨酸（0、0.1、0.2、0.4、0.6g/kg），使日粮中SIDTrp/Lys达到0.12、0.15、0.18、0.21、0.24。每种处理6栏，每栏3头育肥母猪。试验结束时，每栏选取1头育肥母猪，共计30头屠宰后评价胴体性状和肉质（体重121kg）。SIDTrp/Lys增加可以提高日增重（线性和二次效应，P＜0.05），同时改善G:F（线性和二次效应，P＜0.05）。血清尿素氮含量随比值的增加而降低（线性和二次效应，P＜0.05）。处理对背长肌大理石纹和L*light有二次效应（P＜0.05）。SIDTrp/Lys的增加还会提高血浆中生长激素的含量（二次效应，P＜0.05），同时提高血浆中IGF-1的水平（线性和二次效应，P＜0.05）。提高SIDTrp/Lys可以增加背最长肌中钠依赖中性氨基酸转运载体2（SNAT2）的蛋白质丰度（线性和二次效应，P＜0.05）。最大化日增重和G:F，最小化血浆尿素氮水平的合适的SID色氨酸:赖氨酸值在折线模型下分别为0.16、0.17和0.16，在二次方程模型下分别为0.20、0.20、0.20。色氨酸可影响饲喂低蛋白日粮的育肥母猪的血浆生长激素、IGF-1，以及背最长肌SNAT2氨基酸转运载体的蛋白质丰度。
The appropriate standardized ilealdigestible tryptophan to lysine ratio improves pig performance and regulateshormones and muscular amino acid transporters in late finishing gilts fedlow-protein diets
W. F. Ma, S. H. Zhang, X. F. Zeng, X. T.Liu, C. Y. Xie, G. J. Zhang and S. Y. Qiao
This study investigated the effects ofvarious standardized ileal digestible (SID) Trp to Lys ratios on theperformance and carcass characteristics of late finishing gilts receivinglow-CP (9.6%) diets supplemented with crystalline AA. Ninety gilts (89.1 ± 5.1kg) were used in a dose–response study conducted for 35 d. Crystalline Trp (0,0.1, 0.2, 0.4, or 0.6 g/kg) was added to a corn–wheat bran basal diet providingSID Trp to Lys ratios of 0.12, 0.15, 0.18, 0.21, or 0.24. Each diet was fed to6 pens of pigs with 3 gilts per pen. At the end of the experiment, 30 gilts (1 pigper pen) were slaughtered to evaluate carcass traits and meat quality (BW = 121kg). Increasing the SID Trp to Lys ratio increased ADG (linear and quadraticeffect, P < 0.05) and also improved G:F (linear and quadratic effect, P <0.05). Serum urea nitrogen (SUN) decreased as the SID Trp to Lys ratioincreased (linear and quadratic effects, P < 0.05). A quadratic effect of L*light and marbling in the longissimus dorsi was observed as the dietary SID Trpto Lys ratio increased (P < 0.05). Increasing the SID Trp to Lys ratioincreased the level of serum GH (quadratic effect, P < 0.05) and alsoincreased the level of serum IGF-1 (linear and quadratic effect, P < 0.05).Increasing the SID Trp to Lys ratio increased the protein abundance of themuscular AA transporter of sodium-coupled neutral amino acid transporter 2(SNAT2) in the longissimus dorsi muscle (linear and quadratic effect, P <0.05). The optimum SID Trp to Lys ratios to maximize ADG and G:F as well as tominimize SUN levels were 0.16, 0.17, and 0.16 using a linear-breakpoint modeland 0.20, 0.20, and 0.20 using a quadratic model. Tryptophan could influenceserum GH and IGF-1 secretion and protein abundance of the muscular AAtransporter of SNAT2 in the longissimus dorsi muscle in late finishing giltsfed low-protein diets.
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