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Wei Sheng Wu Xue Bao. 2007 Jun 4;47(3):435-40. Links
[Isolation and identification of a lactate-utilizing, butyrate-producing bacterium and its primary metabolic characteristics]
[Article in Chinese]
Liu W, Zhu WY, Yao W, Mao SY.
Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. weil998@sina.com
The distal mammalian gut harbors prodigiously abundant microbes, which provide unique metabolic traits to host. A lactate-utilizing, butyrate-producing bacterium, strain LB01, was isolated from adult swine feces by utilizing modified Hungate technique with rumen liquid-independent YCFA medium supplemented with lactate as the single carbon source. It was an obligate anaerobic, Gram positive bacterium, and could utilize glucose, fructose, maltose and lactate with a large amount of gas products. 16S rRNA sequence analysis revealed that it had the high similarity with members of the genus Megasphaera. The metabolic characteristics of strain LB01 was investigated by using in vitro fermentation system. Lactate at the concentration of 65 mmol/L in YCFA medium was rapidly consumed within 9 hours and was mainly converted to propionate and butyrate after 24h. As the level of acetate declined, the concentration of butyrate rose only in the presence of glucose, suggesting that butyrate could possibly be synthesized by the acetyl CoA: butyryl CoA transferase. When co-cultured with lactic acid bacteria strain K9, strain LB01 evidently reduced the concentration of lactate produced by strain K9 and decelerated the rapid pH drop, finally producing 12.11 mmol/L butyrate and 4.06 mmol/L propionate. The metabolic characteristics that strain LB01 efficiently converts toxic lactate and excessive acetate to butyrate can prevent lactate and acetate accumulation in the large intestine and maintain the slightly acidic environment of the large intestine, consequently revealing that stain LB01 could act as a potential probiotics.
PMID: 17672301 [PubMed - in process]
: J Gen Appl Microbiol. 2007 Apr;53(2):111-7. Links
Selection of Lactobacillus sp. PSC101 that produces active dietary enzymes such as amylase, lipase, phytase and protease in pigs.
Kim EY, Kim YH, Rhee MH, Song JC, Lee KW, Kim KS, Lee SP, Lee IS, Park SC.
College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
This study was carried out to screen lactic acid bacteria that produce active dietary enzymes, such as amylase, lipase, phytase, and protease, using a two-step process in pigs. We isolated a total of 210 and 132 strains of bacteria, grown under aerobic and anaerobic conditions, respectively, in Man Rogosa Sharpe agar containing 0.13% bile after treatment of intestinal samples at pH 3 for 30 min. From fecal samples, a total of 134 aerobic and 111 anaerobic strains were isolated in the same manner. In the second screening test, we selected four strains that produced four dietary enzymes from isolates obtained in the first screening test. Each strain was characterized as lactobacilli based on the following criteria: rod shape, negative for catalase, Gram positive, and lack of acute oral toxicity in mice. Of these four strains, we finally selected Lactobacillus spp. PSC101, which was resistant to pH 3 for 8 h and grew in the presence of 1% bile. In summary, Lactobacillus sp. PSC101 may be a strong probiotic candidate in swine due to its resistance to both acid and bile, its production of dietary enzymes that promote animal growth, and its non-toxic nature in mice.
PMID: 17575451 [PubMed - indexed for MEDLINE]
: Curr Microbiol. 2007 Sep;55(3):260-5. Epub 2007 Jul 25. Links
Probiotic strains and their combination inhibit in vitro adhesion of pathogens to pig intestinal mucosa.
Collado MC, Grześkowiak Ł, Salminen S.
Functional Foods Forum, University of Turku, Turku, Finland. marcol@utu.fi
The aim of this study was to investigate in vitro the protective effect of commercial probiotic strains (Bifidobacterium lactis Bb12 and Lactobacillus rhamnosus LGG) alone and in combination on the adhesion of pathogenic strains as Salmonella, Clostridium, and Escherichia coli to pig intestinal mucus obtained from different intestinal regions. In combination, probiotic strains enhanced each other's adhesion, mainly in large intestinal mucus. Treatment of intestinal mucus with Bb12 and LGG, alone or in combination, significantly reduced (P < 0.05) the adhesion of the tested pathogens. The ability to inhibit pathogen adhesion appears to depend on the specific probiotics and pathogens and on the mucosal site. B. lactis Bb12 and L. rhamnosus LGG in combination revealed a better ability to inhibit adhesion of all pathogens tested to pig intestinal mucus than probiotic strains. Probiotic combinations could be useful for counteracting disease-associated aberrations in intestinal microbiota. Specific protective probiotics could be selected for particular pig pathogens. Probiotic strains from human origin and intended for human use also adhere to pig intestinal mucus and are able to displace and inhibit pathogens.
PMID: 17657533 [PubMed - indexed for MEDLINE]
1: Arch Anim Nutr. 2007 Aug;61(4):223-34. Links
Impact of the probiotic bacteria Enterococcus faecium NCIMB 10415 (SF68) and Bacillus cereus var. toyoi NCIMB 40112 on the development of serum IgG and faecal IgA of sows and their piglets.
Scharek L, Guth J, Filter M, Schmidt MF.
Institut für Immunologie und Molekularbiologie, Freie Universität Berlin, Germany.
To examine the influence of two different probiotic bacteria on the humoral immune system of swine, two animal studies were carried out with sows and their litters. The sows' feed was supplemented with either Enterococcusfaecium NCIMB 10415 (SF68) or Bacillus cereus var. toyoi NCIMB 40112 beginning early in pregnancy. The total IgA content in the faeces as well as the total IgG concentration in the blood of the sows was recorded before and after weaning. The same parameters were determined in the blood and faeces of the piglets. In sows, only feed supplementation with B. cereus led to a clear increase in faecal IgA. Serum IgG levels were not significantly affected by any probiotic feeding in sows. In piglets, the group that was fed B. cereus showed significantly higher faecal IgA levels shortly before weaning, whereas in the E. faecium group, a significant decrease in IgA levels was observed one week after weaning. In both probiotic fed groups the post-weaning IgG levels were significantly decreased compared to the respective control groups. We conclude that B. cereus var. toyoi feed supplementation led to an increased intestinal IgA secretion both in sows and piglets. This effect could be related to a more successful mucosal defence which in turn led to a lower level in systemic IgG production in piglets after weaning.
PMID: 17760301 [PubMed - indexed for MEDLINE]
Asia Pac J Clin Nutr. 2007;16 Suppl 1:143-7. Links
Effect of polysaccharides of cassiae seeds on the intestinal microflora of piglets.
Deng ZY, Zhang JW, Li J, Fan YW, Cao SW, Huang RL, Yin YL, Zhong HY, Li TJ.
Key Laboratory of Food Science, Department of Food Science and Engineering, Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, PR China. dengzy28@yahoo.com.cn
The objective of this study was to examine if polysaccharides from Cassiae Seeds (PCS) can be used as prebiotics to improve the intestinal microflora of piglets with an in vitro and an in vivo trial. The in vitro trial was conducted to study the dose-response effect of PCS on the growth of E. coli 09 and Lactobacillus with traditional plate count method. The gradient culture mediums, containing 3.2, 1.6, 0.8, 0.4, 0.2, 0.1, 0.05, 0.025 and 0% PCS, were inoculated with E. coli09, Lactobacillus and cecum content, respectively. PCS had no influence on the growth of E. coli09 from rejuvenation fluid, but inhibited the growth of E. coli09 from cecum content when the concentration of PCS was higher than 0.1%. Lactobacillus counts were significantly increased with 0.1% PCS or higher (p< 0.05); and the largest increase was found with 0.8% PCS. With the inoculum of cecum content in the medium, Lactobacillus counts increased when the concentration of PCS was 0.4% and 0.8%, whilst E. coli 09 counts decreased. The in vivo trial was carried out to investigate the effect of PCS on the growth of E. coli 09 and Lactobacillus in piglets. Thirty six barrows (average initial BW = 6.5 kg) were randomly divided into 3 groups with 6 each, fed diets supplemented without or with 0.4% or 0.8% PCS. After 14 days, 3 piglets were slaughtered from each group; digesta samples were collected from the ileum, cecum and colon for detection of E.coli 09 and Lactobacillus with plate count method. Samples of the tissue and content of the cecum were taken for detection of caecal microflora profiles with Denaturing Gradient Gel Electrophoresis (DGGE) technique. The dietary inclusion of PCS increased Lactobacillus counts, but reduced E. coli 09 counts in digesta of ileum, cecum and colon of piglets. The dietary inclusion of 0.8% PCS significantly increased the number of electrophoresis brands of caecal bacterial microflora in mucosa and content of the cecum (p< 0.05). These results confirmed the dynamic change in the intestinal microflora profile with the dietary inclusion of PCS in piglets. Thus, PCS can be used as prebiotics to improve the intestinal microflora.
PMID: 17392093 [PubMed - indexed for MEDLINE]
1: Proc Nutr Soc. 2007 May;66(2):260-8. Links
Nutritional management of gut health in pigs around weaning.
Lallès JP, Bosi P, Smidt H, Stokes CR.
INRA, UMR1079, Rearing Systems, Animal and Human Nutrition, F-35590 Saint-Gilles, France. Jean-Paul.Lalles@rennes.inra.fr
Early weaning of piglets is often accompanied by a severe growth check and diarrhoea. It is well established that this process is multi-factorial and that post-weaning anorexia and undernutrition are major aetiological factors. Gastrointestinal disturbances include alterations in small intestine architecture and enzyme activities. Recent data indicate transiently-increased mucosal permeability, disturbed absorptive-secretory electrolyte balance and altered local inflammatory cytokine patterns after weaning. These responses appear to operate according to two distinct temporal patterns, an acute response followed by a long-lasting adaptation response. Pigs coexist with a diverse and dense commensal microbiota in their gastrointestinal tract. Most of these microbes are beneficial, providing necessary nutrients or protection against harmful pathogens for the host. The microbial colonisation of the porcine intestine begins at birth and follows a rapid succession during the neonatal and weaning period. Following the withdrawal of sow's milk the young piglets are highly susceptible to enteric diseases partly as a result of the altered balance between developing beneficial microbiota and the establishment of intestinal bacterial pathogens. The intestinal immune system of the newborn piglet is poorly developed at birth and undergoes a rapid period of expansion and specialisation that is not achieved before early (commercial) weaning. Here, new insights on the interactions between feed components, the commensal microbiota and the physiology and immunology of the host gastrointestinal tract are highlighted, and some novel dietary strategies are outlined that are focused on improving gut health. Prebiotics and probiotics are clear nutritional options, while convincing evidence is still lacking for other bioactive substances of vegetable origin.
PMID: 17466106 [PubMed - indexed for MEDLINE]
1: Curr Issues Intest Microbiol. 2007 Mar;8(1):1-7. Links
Effect of the probiotic Enterococcus faecium NCIMB10415 on cell numbers of total Enterococcus spp., E. faecium and E. faecalis in the intestine of piglets.
Vahjen W, Taras D, Simon O.
Institute of Animal Nutrition, Free University of Berlin, Bruemmerstr. 34, 14195 Berlin, Germany. vahjen@zedat.fu-berlin.de
Sows and their piglets were fed a diet supplemented with or without the probiotic E. faecium NCIMB10415 (also known as SF68). Piglets were sacrificed 14, 28, 35 and 56 days after birth and DNA from intestinal segments was extracted and purified. A real time PCR assay was used to distinguish Enterococcus spp. (16s rDNA based), E. faecium (Efaafm gene), E. faecalis (Efaafs gene) as well as the probiotic strain (unique plasmid sequence). Extracts of autoclaved sow feces inoculated with E. faecium and E. faecalis cultures were used to calibrate real time PCR results. The probiotic strain was detected in 14 day old suckling piglets before the piglets had access to the starter diet. In piglets of the probiotic group, probiotic E. faecium cell counts were always a significant proportion of total E. faecium cells in stomach digesta (4-20%), however only a small fraction of the total Enterococcus spp. cell number on day 14 and 28 in all intestinal segments (0.1-0.7%). Compared to control samples, the probiotic E. faecium strain significantly (p < or = 0.05) decreased the amount of total Enterococcus spp. and E. faecalis cells in the colon of 14 day old suckling piglets as well as in jejunum and colon samples one week after weaning. E. faecium cell counts were not modified on any sampling day or intestinal segment. This study showed that the presence of probiotic E. faecium NCIMB10415 coincided with reduced total E. faecalis, but not total E. faecium cell numbers in the intestine of piglets. In view of unchanged cell numbers and ratios in sow feces, modifications must have taken place within the intestine of suckling piglets.
PMID: 17489433 [PubMed - indexed for MEDLINE]
1: Vet Microbiol. 2007 Oct 6;124(3-4):358-61. Epub 2007 Apr 8. Links
Prevention of porcine Clostridium difficile-associated disease by competitive exclusion with nontoxigenic organisms.
Songer JG, Jones R, Anderson MA, Barbara AJ, Post KW, Trinh HT.
Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, AZ 85721, USA. gsonger@u.arizona.edu
Clostridium difficile is widely known as a cause of disease in humans, and has emerged as an important problem in neonatal swine. No commercial product is available for immunoprophylaxis of C. difficile-associated disease, but success in preventing experimental infections in hamsters by use of nontoxigenic strains to competitively exclude toxigenic strains led us to try this method in neonatal pigs. Spores were administered orally to newborn pigs or were sprayed onto perineum and teats of dams. Significantly more piglets were weaned among litters receiving spores orally, and average weaning weights were significantly higher for both treatment groups than for controls. Toxins A and B were detected in 44.8% of litters and 16.5% of piglets born to sprayed sows and 58.3% of litters and 15.4% of piglets in the control group. However, toxins were detected in only 13.8% of litters and 3.4% of piglets given spores orally. These data support a contention that precolonization by a nontoxigenic strain can ameliorate the pre-weaning growth retardation associated with C. difficile infection in piglets.
PMID: 17493774 [PubMed - indexed for MEDLINE]
Bacillus cereus var. toyoi enhanced systemic immune response in piglets.
Schierack P, Wieler LH, Taras D, Herwig V, Tachu B, Hlinak A, Schmidt MF, Scharek L.
Institut für Mikrobiologie und Tierseuchen, FU Berlin, Philippstrasse 13, 10115 Berlin, Germany. schierack.peter@vetmed.fu-berlin.de
Probiotic bacteria have been suggested to stimulate the host immune system. In this study we evaluated the immunomodulatory effects of probiotic Bacillus cereus var. toyoi on the systemic immunity of piglets. A pool of 70 piglets was divided into a probiotic or control group. We determined the ratios of peripheral blood mononuclear cell (PBMC) subsets and measured proliferative responses and cytokine production of PBMCs and effects on vaccination responses. Blood samples of probiotic-treated piglets showed a significantly lower frequency of CD8(high)/CD3+ T cells and CD8(low)/CD3+ T cells and a significant higher CD4+/CD8+ ratio. IL-4 and IFN-gamma production of polyclonally stimulated PBMCs was on average higher in the probiotic group. Specific proliferative responses of PBMCs to Influenza vaccination antigens were significantly higher and antibody titers against H3N2 Influenza and Mycoplasma vaccination antigens were on average higher in the probiotic group. In conclusion, B. cereus var. toyoi therefore alters the immune status of piglets as indicated by changes in the ratios as well as functionalities of systemic immune cell populations.
PMID: 17498814 [PubMed - indexed for MEDLINE]
1: Antiviral Res. 2007 Sep;75(3):266-74. Epub 2007 Apr 23. Links
Interactions of macrophages with probiotic bacteria lead to increased antiviral response against vesicular stomatitis virus.
Ivec M, Botić T, Koren S, Jakobsen M, Weingartl H, Cencic A.
University of Maribor, Faculty of Agriculture, Vrbanska c.30, 2000 Maribor, Slovenia.
Macrophages are an important cellular component of the innate immune system and are normally rapidly recruited and/or activated at the site of virus infection. They can participate in the antiviral response by killing infected cells, by producing antiviral cytokines such as nitric oxide and by producing chemokines and immunoregulatory cytokines that enable the adaptive immune response to recognize infected cells and perform antiviral effector functions. Probiotics, as a part of the normal gut intestinal flora, are important in supporting a functional yet balanced immune system. Improving our understanding of their role in the activation of macrophages and their stimulation of proinflammatory cytokine production in early viral infection was the main goal of this study. Our in vitro model study showed that probiotic bacteria, either from the species Lactobacillus or Bifidobacteria have the ability to decrease viral infection by establishing the antiviral state in macrophages, by production of NO and inflammatory cytokines such as interleukin 6 and interferon-gamma. These effects correlated with the mitochondrial activity of infected macrophages, therefore, the measurements of mitochondrial dehydrogenases activity could be implied as the first indicator of potential inhibitory effects of the probiotics on virus replication. The interactions between probiotic bacteria, macrophages and vesicular stomatitis virus (VSV), markedly depended on the bacterial strain studied.
PMID: 17512614 [PubMed - indexed for MEDLINE]
1: Anaerobe. 2007 Jun-Aug;13(3-4):107-13. Epub 2007 Apr 25. Links
Different probiotic properties for Lactobacillus fermentum strains isolated from swine and poultry.
Lin WH, Yu B, Jang SH, Tsen HY.
School of Pharmacy, China Medical University, No. 91, Hsueh Shih Rd., Taichung City 404, Taiwan, ROC.
Systematic procedures were used to evaluate the probiotic properties of Lactobacillus fermentum (L. fermentum) strains isolated from swine and poultry. The major properties included their capabilities to adhere to the intestinal epithelium of swine and poultry, the inhibition on pathogenic bacteria, and their tolerance to the gastric juice and bile salts. Results showed that L. fermentum strains from poultry digestive tract showed better adherence to the swine intestine and chicken crop epithelial cells as compared to those strains from the swine origin. In addition, six strains from poultry and one strain from swine showed adhesion specificity to their own intestinal epithelium. Four poultry isolates and one swine isolate were able to adhere to the epithelial cells from both swine and chicken. For gastric juice and bile tolerance, most of the strains isolated from swine or poultry were acid tolerant but less strains were bile intolerant. The spent culture supernatant (SCS) of these L. fermentum strains showed antagonistic effect against the indicator bacteria, such as Escherichia coli, Salmonella spp., Shigella sonnei and some enterotoxigenic Staphylococcus aureus. From the above studies, some L. fermentum strains isolated from poultry were found to have the probiotic properties required for use in animal feed supplement. This study suggested that poultry digestive tract may serve as potential source for the isolation of probiotic lactic acid bacteria.
PMID: 17544731 [PubMed - indexed for MEDLINE]
1: Berl Munch Tierarztl Wochenschr. 2007 May-Jun;120(5-6):221-5. Links
Effect on the immune system of germ-free piglets of probiotics potentiated with polyunsaturated fatty acids.
Kastel R, Bomba A, Herich R, Vasko L, Svedova M.
Department of Chemistry, Biology and Biochemistry, University of Veterinary Medicine Kosice, Slovak Republic. kastel@uvm.sk
The present study investigated the influence of polyunsaturated fatty acids (PUFA) on the immune system of germ-free piglets. Oil with increased content of omega-3 PUFA was administered to piglets from the experimental group (EG) for four weeks. Piglets from the control group (CG) received identical volumes of saline solution. At the age of 21 days both groups of germ-free piglets were inoculated perorally with Lactobacillus casei subsp. casei at a dose of 2 ml (1x10(8) mli). At the age of 28 days, i.e. after one-week colonisation of germ-free piglets with Lactobacillus casei subsp. casei, significant differences were recorded in phagocytic activity of neutrophils (PANe) and phagocytic activity of potentially phagocytizing cells (PA) (P < 0.05). Between EG and CG there have been observed no significant differences in absolute numbers of CD4+ and CD8+ T lymphocytes and numbers of IgM cells and in additional investigated parameters - number of CD2+ T lymphocytes, index of phagocytic activity of neutrophils (IPANe) and index of phagocytic activity (IPA).The total number of leukocytes (Le) in EG was also higher. Of the parameters determined in blood serum we observed a significant increase in concentration of alpha linolenic, eicosapentaenoic and docosahexaenoic acids and a parallel decrease in the level of arachidonic acid.
PMID: 17555043 [PubMed - indexed for MEDLINE]
1: Vet Microbiol. 2007 Jul 20;123(1-3):133-44. Epub 2007 Feb 24. Links
Survival and persistence of Lactobacillus plantarum 4.1 and Lactobacillus reuteri 3S7 in the gastrointestinal tract of pigs.
De Angelis M, Siragusa S, Caputo L, Ragni A, Burzigotti R, Gobbetti M.
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari, Bari, Italy. m.deangelis@agr.uniba.it
Lactobacillus sp. are important inhabitants of the intestines of animals. They are also largely used as probiotics for both humans and animals. To exert beneficial effects, lactobacilli have to survive through the gastrointestinal transit. Based on bile-salt resistance, pH tolerance, antimicrobial activity and heat resistance, Lactobacillus plantarum 4.1 and Lactobacillus reuteri 3S7 were previously selected and used as probiotic additives in pelleted feeding trials. Both strains were fed to pigs (sows and piglets) at a cell number of ca. 10(10) viable cells per day. A polyphasic approach, comprising growth on selective media, Biolog System analysis, 16S rRNA gene sequencing and RAPD-PCR typing, was used to identify and differentiate L. plantarum 4.1 and L. reuteri 3S7 from other faecal Lactobacillus sp., L. plantarum 4.1 and L. reuteri 3S7 had the capacity to survive during the gastrointestinal transit and were found in the feaces at a cell number of 6-8 log cfu/g. Their persistence was shown after 6 days from the administration. Compared to untreated pigs, the administration of L. plantarum 4.1 and L. reuteri 3S7 significantly (P<0.05) decreased the population of Enterobacteriaceae. Besides, the beta-glucuronidase activity of all pigs decreased of ca. 23.0% during administration. The findings of this study showed that L. plantarum 4.1 and L. reuteri 3S7 have the potential to be used as probiotic additives in pelleted feed for pigs.
PMID: 17391871 [PubMed - indexed for MEDLINE]
1: Antonie Van Leeuwenhoek. 2006 Aug;90(2):139-46. Epub 2006 Jul 4. Links
Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs.
Guo X, Li D, Lu W, Piao X, Chen X.
National Key Laboratory on Animal Nutrition, China Agricultural University, No. 2. West Road Yuanmingyuan, Beijing 100094, PR China.
A total of 124 samples were collected from the intestine of broiler chickens, piglet faeces, fermented foods, soils and Chinese herbs. More than 750 strains of aerobic, spore-forming bacteria were isolated from these samples. The inhibitory activity of these spore-forming strains against Escherichia coli K88, E. coli K99, Salmonella typhimurium and Staphylococcus aureus was assessed using a disc plate diffusion assay. The six bacilli with the largest inhibition zones against the four indicator bacteria were chosen and assessed for their resistance to unfavorable conditions within simulated gut environments. The strain Bacillus subtilis MA139 showed full resistance to pH 2, 0.3% bile salts and exhibited the highest antimicrobial activity. Based on these results, B. subtilis MA139 was selected as a potential probiotic and fed to piglets at concentrations of 2.2 x 10(5), 2.2 x 10(6) or 2.2 x 10(7) CFU/g of feed during a 28-day feeding trial. A negative control consisting of the basal diet with no additives and a positive control consisting of the basal diet supplemented with 16 g/ton flavomycin were also included. Ninety piglets between 35 and 40 days old were used in the in vivo animal trials. B. subtilis MA139 enhanced daily gain (P = 0.10) and feed conversion (P = 0.03) compared with the negative control. The performance of pigs fed B. subtilis MA139 supplemented diets did not differ from that of pigs fed the antibiotic diet. There was a significant increase in Lactobacilli cell counts (P = 0.02) and a numerical decrease in E. coli counts (P = 0.05) in the faecal samples of pigs fed B. subtilis MA139 with 2.2 x 10(5) CFU/g of feed. The overall results of this study show that the use of initial co-culture with indicator pathogens, a disc plate diffusion assay and simulated gut environment tolerance tests is one of effective methods of screening Bacillus for probiotic use and that B. subtilis MA139 is a promising alternative to antibiotics for use as a feed additive in piglet diets.
PMID: 16820971 [PubMed - indexed for MEDLINE]
1: Res Microbiol. 2006 Oct;157(8):792-801. Epub 2006 Jun 23. Links
Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding.
De Angelis M, Siragusa S, Berloco M, Caputo L, Settanni L, Alfonsi G, Amerio M, Grandi A, Ragni A, Gobbetti M.
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di Agraria, Università degli Studi di Bari, Bari, Italy.
Thirty-five isolates from pig feces were identified as Lactobacillus reuteri (12 strains), Lactobacillus mucosae (7), Lactobacillus plantarum (6), Lactobacillus kitasatonis (3), Lactobacillus rossiae (2), Lactobacillus ultunensis (2), Lactobacillus crispatus (2), and Lactobacillus intestinalis (1) by partial sequence analysis of the 16S rRNA. All isolates were detected at 8-9 log CFU g(-1). Preliminarily, strains were selected based on resistance to heat treatments (ca. 70 degrees C for 10 s). The decrease in viability for some L. reuteri, L. mucosae, L. plantarum, L. kitasatonis, and L. rossiae strains was lower than 1 log cycle. Selected strains were further characterized for acid and bile salt resistance, and antibacterial activity. Except for L. kitasatonis, tolerance to simulated gastric and intestinal conditions was enhanced for all strains by addition of reconstituted skimmed milk. Antibacterial activity was found against Gram-positive and -negative potential pathogens. L. reuteri 8.1, 3S7, 6.2, and 1.2, L. mucosae 1.1R, L. plantarum 4.1, and L. rossiae 4.4 were freeze-dried and mixed (1%, w/w) into pig feed before pelleting. After pelleting, pig feed contained 10-9 log CFU kg(-1) of lactobacilli. L. plantarum 4.1, and L. reuteri 3S7 were selected based on their bile salt resistance, pH tolerance, antimicrobial activity and heat resistance. The findings in this study provide a strong basis for exploring the potential of porcine lactobacilli isolates to be used in pelleted feeding as probiotic additives.
PMID: 16844349 [PubMed - indexed for MEDLINE]
1: J Anim Physiol Anim Nutr (Berl). 2006 Aug;90(7-8):309-15. Links
Dietary probiotic supplementation and resulting effects on performance, health status, and microbial characteristics of primiparous sows.
Böhmer BM, Kramer W, Roth-Maier DA.
Division of Animal Nutrition, Technical University Munich, Hochfeldweg 6, D-85350 Freising-Weihenstephan, Germany.
In an experiment with 33 first-litter sows from day 90 of pregnancy to day 28 of lactation, the influence of a probiotic supplementation on weight performance, feed intake, litter sizes, litter weights, health status and microbiological profile was tested. Enterococcus faecium DSM 7134 was supplemented in a concentration of 5 x 10(8) CFU/kg feed to the gestation and lactation diets of gilts. The supplemented sows showed a significant higher improvement of feed intake (4.16 vs. 3.71 kg/day), litter size (9.2 vs. 7.7 piglets) and weight performance. The average live weight of the probiotic sows at day 28 of lactation was 11 kg higher than of the controls. The bacterial counts/g faeces (lactobacilli, Gram-positive anaerobes, Gram-negative anaerobes, Escherichia coli and enterococci) and the incidence of adhesive and haemolytic E. coli organisms revealed no significant differences between the sows of the two groups or their piglets. While the litter size cannot necessarily be assumed as a primary effect of the probiotic supplementation, the significantly better feed intake and weight performance might be partly due to the probiotic use and can prevent "starvation sterility" of young sows after their first litter caused by reduced feed intake during lactation with high mobilization of body tissue accompanied with lack of energy.
PMID: 16867076 [PubMed - indexed for MEDLINE]
1: Arch Anim Nutr. 2006 Aug;60(4):277-88. Links
Effects of live yeast on the performance, nutrient digestibility, gastrointestinal microbiota and concentration of volatile fatty acids in weanling pigs.
Li J, Li D, Gong L, Ma Y, He Y, Zhai H.
National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing.
Two experiments were conducted to evaluate the effects of live yeast supplementation on performance, nutrient digestibility, enteric microbial populations and volatile fatty acid (VFA) concentration of weanling pigs, receiving diets supplemented with aureomycin and elevated doses of CuSO4. In experiment 1, 90 crossbred pigs (7.20 +/- 0.44 kg, 28 d of age) were randomly allotted to one of five dietary treatments containing either 0, 4.0 x 10(6), 9.0 x 10(6), 2.6 x 10(7), or 5.1 x 10(7) cfu Saccharomyces cerevisiae per gram with six replicate pens per treatment and three pigs per pen. BWG and feed intake increased quadratically during days 1-14 and days 1-28 as live yeast levels increased (p < 0.01). Pigs fed the diet containing 2.6 x 10(7) cfu yeast per gram had the highest BWG and feed intake among the treatments. In experiment 2, 48 crossbred pigs (7.64 +/- 0.72 kg, 28 d of age) were fed diets containing live yeast at 0 or 3.2 x 10(7) cfu of S. cerevisiae per g with six replicate pens per treatment and four pigs per pen. The yeast supplementation improved BWG and feed intake during days 1-14 (p < 0.01) and days 1-28 (p < 0.05). Treatment differences were not observed in any of the bacterial populations, yeast numbers or VFA concentrations, at any of the sites of the gastrointestinal tract tested. Total tract nutrient digestibility was also not different between treatments. Overall, dietary supplementation of live yeast had a positive effect on BWG and feed intake of weanling pigs, receiving diets supplemented with aureomycin and elevated doses of CuSO4. The improvement in BWG appears to be partly related to an increase in feed intake. The mechanism of yeast improving feed intake of piglets needs to be explored.
PMID: 16921925 [PubMed - indexed for MEDLINE]
1: Vet Microbiol. 2007 Jan 31;119(2-4):248-55. Epub 2006 Oct 16. Links
Effect of orally administered Lactobacillus casei on porcine reproductive and respiratory syndrome (PRRS) virus vaccination in pigs.
Kritas SK, Morrison RB.
Department of Microbiology and Infectious Diseases/School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Macedonia, Greece. skritas@vet.auth.gr
The purpose of this study was to determine whether intranasal/oral administration of probiotics can assist vaccination efficacy against an important swine pathogen, porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV). A controlled challenge trial was performed employing: (a) pigs vaccinated against PRRS and treated with a Lactobacillus casei, (b) pigs vaccinated against PRRS only, (c) pigs treated with L. casei only, and (d) pigs neither vaccinated against PRRS nor treated with L. casei. All pigs were challenged intranasally with a wild PRRSV strain. There was no difference in clinical signs or rectal temperature among the four groups. However, pigs that received L. casei gained significantly more weight than pigs that did not. Vaccinated pigs did not gain more weight than nonvaccinated pigs. Vaccinated groups had significantly fewer viraemic pigs on days post-challenge 4, 11 and 17 than nonvaccinated groups of pigs. There was no effect of probiotic on prevalence or duration of viraemia. Among viraemic pigs, there was no significant difference in mean log base(10) titer of PRRS virus among groups. These results suggest that orally administered L. casei does not affect immune response in such a way as to affect PRRS viraemia or nasal shedding. However, it still appears to provide significant benefit when administered during vaccination as indicated by the higher bodyweight gain following PRRS virus infection.
PMID: 17049424 [PubMed - indexed for MEDLINE]
1: Rev Latinoam Microbiol. 2004 Jan-Jun;46(1-2):5-11. Links
Resistance of Enterococcus strains isolated from pigs to gastrointestinal tract and antagonistic effect against Escherichia coli K88.
García-Galaz A, Pérez-Morales R, Díaz-Cinco M, Acedo-Félix E.
Centro de Investigación en Alimentación y Desarrollo, Sonora, México.
The intestinal flora plays an important role in health and wellbeing of different organisms. Indigenous microflora can be innocuous or pathogenic. Consumption of food supplemented with beneficial microorganisms as probiotics provides a good health state and this can be maintained and recovered. Currently, probiotic strains of Bifidobacterium and Lactobacillus are widely used in humans as well as animals. Swine industry would benefit with the application of probiotics, mainly to overcome diarrheal diseases produced by different causes, as a pathogenic E. coli K88. The aim of this work was to isolate strains of Enterococcus from gastrointestinal tract of pigs to use them as probiotic. Two strains of E. faecalis, 2 of E. mundii and 7 of E. faecium were isolated with characteristics of resistance to acid pH, tolerance to biliary salts and a high antagonistic activity (>80%) against E. coli K88. Based on their characteristics and species affinity, we believe that these strains could be administered to pigs as a probiotic.
PMID: 17061520 [PubMed - indexed for MEDLINE]
1: Rev Latinoam Microbiol. 2005 Jul-Dec;47(3-4):70-5. Links
In vitro evaluation of the binding capacity of Saccharomyces cerevisiae Sc47 to adhere to the wall of Salmonella spp.
Pérez-Sotelo LS, Talavera-Rojas M, Monroy-Salazar HG, Lagunas-Bernabé S, Cuarón-Ibargüengoytia JA, Jimenez RM, Vázquez-Chagoyán JC.
Centro de Investigaciones y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México. luismsp09@hotmail.com.mx
Saccharomyces cerevisiae Sc47 (Biosaf) is a commercially available baker's yeast strain (Lesaffre, France) that has been used as a probiotic in animal nutrition. It has been previously reported that animals fed with the yeast showed an improved resistance to several enteric infectious diseases. Some of the S. cerevisiae strains adhere potentially pathogenic bacteria such as Escherichia coli and Salmonella spp. This could be a mechanism through which animals fed with the yeast may become more resistant to infections caused by these microorganisms. In this paper, the adhesion of forty-five Salmonella spp. isolates to Sc47 was assessed by sedimentation and agglutination tests, and by light and electron microscopy. Results showed that 57.7% (26/45) of the isolates and 66.6% (6/9) of the Salmonella serovars tested adhered to the Sc47 cell wall.
PMID: 17061530 [PubMed - indexed for MEDLINE]
1: Anaerobe. 2006 Oct-Dec;12(5-6):242-8. Epub 2006 Oct 27. Links
Enterococcus faecium EK13--an enterocin a-producing strain with probiotic character and its effect in piglets.
Strompfová V, Marcináková M, Simonová M, Gancarcíková S, Jonecová Z, Sciranková L, Koscová J, Buleca V, Cobanová K, Lauková A.
Institute of Animal Physiology, Slovak Academy of Sciences, Soltésovej 4-6, 04001 Kosice, Slovakia. strompfv@saske.sk
The experiment was conducted to determine the effects of the inoculation of the probiotic and enterocin A-producing strain Enterococcus faecium EK13 on selected parameters of metabolic profile, gut microflora, growth, and health in newborn piglets of Slovak White Improved. Piglets for study were divided into two groups: one group (EK13 group, n=8) received strain EK13 per os once daily for 7 days (2ml per piglet, 10(9)CFU/mL of saline buffer). The control group of piglets (n=7) was given placebo-saline buffer. The experiment lasted 14 days. After 7 days, strain EK13 reached 9.8 log(10) CFU/g in faeces of E. faecium EK13 treated piglets while counts of Escherichia coli were significantly lower (P<0.01) than in piglets of the control group. The concentrations of total serum protein, calcium, haemoglobin, haematocrit, red blood cell count and index of phagocytic activity of leukocytes were significantly higher after application of strain EK13. On the other hand, cholesterol was significantly lower in the EK13 group of animals. On day 14, piglets were killed and samples of intestinal contents were taken. Total counts of bacteria in the intestinal contents (jejunum, ileum, caecum, colon) were not significantly influenced. The pH value was significantly lower (P<0.05) only in duodenum of piglets receiving E. faecium EK13. There was a significant higher concentration of lactic acid (P<0.01) and propionic acid in the colon (P<0.001) of the EK13 group. Application of E. faecium EK13 did not influence the daily body weight gain significantly.
PMID: 17071114 [PubMed - indexed for MEDLINE]
1: J Appl Microbiol. 2006 Dec;101(6):1357-66. Links
Detection and distribution of probiotic Escherichia coli Nissle 1917 clones in swine herds in Germany.
Kleta S, Steinrück H, Breves G, Duncker S, Laturnus C, Wieler LH, Schierack P.
Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, Berlin, Germany.
AIMS: To verify the presence of Escherichia coli Nissle 1917 as a natural isolate in swine and to characterize in vitro probiotic properties as well as in vivo persistence in a feeding experiment. METHODS AND RESULTS: During studies on the intestinal microflora of pigs, we isolated E. coli Nissle 1917 sporadically from a pig population over a period of 1 year. The identity of the isolates as E. coli Nissle 1917 was verified by serotyping, Nissle-specific PCR, macrorestriction analysis (pulsed field gel electrophoresis) and the determination of in vitro probiotic properties in invasion and adhesion assays using a porcine intestinal epithelial cell line. Both the E. coli isolates and the E. coli Nissle 1917 strain showed strong reductions in adhesion of porcine enteropathogenic E. coli and invasion of Salmonella typhimurium with epithelial cells in vitro, with a probiotic effect. Screening of five epidemiologically unlinked swine farms and two wild boar groups showed one farm positive for E. coli Nissle 1917. A feeding experiment with four piglets showed viable E. coli Nissle 1917 in the intestine of three animals. CONCLUSIONS: The results of this study suggest that the E. coli Nissle 1917 strain is already partially established in swine herds, but the colonization of individual animals is variable. SIGNIFICANCE AND IMPACT OF THE STUDY: We report natural, long-term colonization and transmission of the probiotic E. coli Nissle 1917 strain in a swine herd, characterized individual persistence and colonization properties in swine and established an in vitro porcine intestinal epithelial cell model of probiotic action. The results of this study would have implications in the use of this strain as a probiotic in swine and contribute to a better understanding of the individual nature of intestinal bacterial persistence and establishment.
PMID: 17105567 [PubMed - indexed for MEDLINE]
1: Anim Biotechnol. 2006;17(2):217-31. Links
Reduced use of antibiotic growth promoters in diets fed to weanling pigs: dietary tools, part 2.
Stein HH, Kil DY.
Department of Animal Sciences, University of Illinois, Urbana, Illinois 61801, USA. hstein@uiuc.edu
Diets formulated to maximize performance of weanling pigs need to support the development of intestinal tissue, support intestinal colonization with beneficial, mainly lactic acid-producing bacteria, and support development of the intestinal and overall immune system. This objective is not likely to be achieved using one single strategy, but there is strong evidence that diets formulated with cereal grains other than corn, with a low concentration of crude protein and with the use of direct-fed microbials, will improve intestinal health and performance of weanling pigs. Further improvements may be observed if the grain part of the diet is fermented prior to feeding or if the diet is fed in a liquid form, but the need for specialized equipment limit the implementation of this strategy. Dietary supplements such as essential oils and nucleosides or nucleotides may also be useful, but more research is needed to verify the effects of these substances.
PMID: 17127532 [PubMed - indexed for MEDLINE]
1: Ann N Y Acad Sci. 2006 Oct;1081:276-9. Links
Characterization of lactic acid bacteria and other gut bacteria in pigs by a macroarraying method.
Thanantong N, Edwards S, Sparagano OA.
School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
Lactic acid bacteria (LAB) consist of many genera, Gram-positive, and nonspore-forming micro-organisms; some members being used as probiotics while some others have negative effects on pig health. Bacterial species in the gastrointestinal tract can produce antibacterial substances, reduce serum cholesterol in their host, or can be responsible for growth reduction, diarrhea, and intestinal epithelial damage. It is therefore important for the pig industry to evaluate the impact of food and farm management on the presence of "good" or "bad" bacteria and the risk for consumers. This articles focuses on the molecular identification of gut microflora species following different diets given to pigs in UK and correlating the data on growth, health, and welfare. First of all, pig feces were individually collected from sows before and after farrowing and also from piglets before and after weaning over several months. Bacteria colonies were grown on MRS agar plates from feces and DNA was extracted (QIAamp DNA stool kit) and amplified using 16S rDNA (27f and 519r) primers. DNA sequencing and sequence alignment allowed us to identify species-specific zones, which were used as probes in a macroarray system also known as reverse line blot hybridization. Some probes were found to be species specific for the following species: Lactobacillus acidophilus, L. animalis, L. gallinarum, L. kitasanotis, L salivarius, Streptococcus alactolyticus, S. hyointestinalis, and Sarcina ventriculi. Actual studies are now focusing on the impact of diets of the microflora in different gut parts and at different stages of the animal's life.
PMID: 17135526 [PubMed - indexed for MEDLINE]
1: Appl Microbiol Biotechnol. 2007 Apr;74(5):1103-11. Epub 2006 Nov 29. Links
Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract.
Kim PI, Jung MY, Chang YH, Kim S, Kim SJ, Park YH.
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, South Korea.
One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.
PMID: 17136367 [PubMed - indexed for MEDLINE]
1: Commun Agric Appl Biol Sci. 2006;71(1):227-30.
Fermented liquid feed for weaned piglets.
Missotten J, Michiels J, Goris J, Herman L, Heyndrickx M, De Smet S, Dierick N.
Laboratory for Animal Nutrition and Animal Product Quality, Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, BE-9090 Melle, Belgium.
PMID: 17191511 [PubMed - indexed for MEDLINE]
1: Appl Environ Microbiol. 2007 Mar;73(6):1858-63. Epub 2007 Jan 19. Links
A five-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged with Salmonella enterica Serovar Typhimurium.
Casey PG, Gardiner GE, Casey G, Bradshaw B, Lawlor PG, Lynch PB, Leonard FC, Stanton C, Ross RP, Fitzgerald GF, Hill C.
Department of Microbiology, University College, College Road, Cork, Ireland. c.hill@ucc.ie
Salmonella spp. infection is a major cause of gastroenteritis, with many thousands of cases reported in the European Union every year. The use of probiotics offers the potential to improve this situation. Here, we investigate the effects of oral treatment of pigs with a defined lactic acid bacteria culture mixture on both clinical and microbiological signs of Salmonella enterica serovar Typhimurium infection. Fifteen weaned pigs blocked by sex and weight were administered control milk or a mixture of five probiotic strains as either a milk fermentate or milk suspension for a total of 30 days. The mixture consisted of two strains of Lactobacillus murinus and one strain each of Lactobacillus salivarius subsp. salivarius, Lactobacillus pentosus, and Pediococcus pentosaceous. Following probiotic administration for 6 days, animals were challenged orally with serovar Typhimurium; the health of the animals and the microbiological composition of their feces were monitored for 23 days postinfection. Animals treated with probiotic showed reduced incidence, severity, and duration of diarrhea. These animals also gained weight at a greater rate than control pigs administered skim milk. Mean fecal numbers of Salmonella were significantly reduced in probiotic-treated animals at 15 days postinfection (P = 0.01). The administered probiotic bacteria improved both the clinical and microbiological outcome of Salmonella infection. These strains offer significant benefit for use in the food industry and may have potential in human applications.
PMID: 17261517 [PubMed - indexed for MEDLINE]
1: Pol J Microbiol. 2005;54(4):331-4. Links
Effect of intensity of feeding on the intestinal microflora of pigs.
Rekiel A, Gajewska J, Topol K, Sawosz E.
Department of Animal Breeding and Husbandry, Faculty of Animal Sciences, Warsaw Agricultural University, Poland.
In individual, single-phase feeding animals were fed extensively (group E--7 animals) or intensively (group I--7 animals) in semi ad libitum system. The mixtures differed in composition as well as energy and nutritional value, with constant ratio of protein to energy of 13.12:1 in intensive feeding and 13.04:1 in extensive feeding. Fibre content per 1 kg mixture was 3.43% in group I and 12.3% in group E. For microbiological studies samples were taken from the duodenum, ileum, jejunum and large intestine and both quantitative and qualitative differences in the microflora of the differently fed groups was found.
PMID: 16599306 [PubMed - indexed for MEDLINE]
1: J Appl Microbiol. 2006 Apr;100(4):838-45. Links
Adhesion of Lactobacillus plantarum 423 and Lactobacillus salivarius 241 to the intestinal tract of piglets, as recorded with fluorescent in situ hybridization (FISH), and production of plantaricin 423 by cells colonized to the ileum.
Maré L, Wolfaardt GM, Dicks LM.
Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa.
AIMS: To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model. METHODS AND RESULTS: Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combination, were administered to 1-, 14- and 28-day-old (postweaned) piglets. According to results obtained by fluorescent in situ hybridization (FISH), Lact. plantarum 423 adhered strongly to the ileum and posterior colon and Lact. salivarius 241 to the duodenum in preweaned piglets. High numbers of strain 241 were recorded in the duodenum and posterior colon of postweaned piglets, whereas strain 423 remained localized to the ileum. Lowering in Enterococcus faecalis cell numbers were recorded when preweaned piglets were challenged with strain 241. Plantaricin 423 was produced for 96 h in the ileum section of a gastro-intestinal model. CONCLUSIONS: Lactobacillus plantarum 423 and Lact. salivarius 241 adhere to different sections of the intestinal tract, depending on the piglet's age. Ent. faecalis were inhibited in vivo, probably by plantaricin 423. SIGNIFICANCE AND IMPACT OF THE STUDY: Fluorescent in situ hybridization proved valuable in the detection of probiotic bacteria adhered to the intestine. This is the first report of bacteriocin production in a model simulating the porcine gastro-intestinal tract.
PMID: 16553740 [PubMed - indexed for MEDLINE]
1: Vet Immunol Immunopathol. 2006 Jun 15;111(3-4):239-50. Epub 2006 Mar 13. Links
Effect of orally administered probiotic E. coli strain Nissle 1917 on intestinal mucosal immune cells of healthy young pigs.
Duncker SC, Lorentz A, Schroeder B, Breves G, Bischoff SC.
Department of Physiology, School of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany. duncker@mcmaster.ca
Several beneficial effects of probiotics have been described in studies using rodent disease models and in human patients; however, the underlying mechanisms remained mostly unclear. Only a few studies focused on the effects of probiotics on the intestinal mucosal immune system. Here, we studied the effect of the probiotic strain E. coli Nissle 1917 (EcN) administered orally to young pigs at two concentrations (10(9) and 10(11)CFU/d for 21 days) on the gut-associated lymphatic tissue. This probiotic strain was shown recently to reduce recurrence of inflammation in ulcerative colitis patients. We quantified the number and distribution of intestinal immune cells (granulocytes, mast cells, CD4+, CD8+, CD25+, IgA+ lymphocytes) and the mucosal mRNA expression of cytokines (IFN-gamma, TNF-alpha, TGF-beta, IL-10) and antimicrobial peptides (PR-39, NK-lysin, prepro-defensin-beta 1, protegrins). The number and distribution of cells were highly different between small intestinal and colon segments in all groups, but were not influenced by EcN, except high dose EcN fed pigs (10(11) CFU/d) showing an increase in mucosal CD8+ cells in the ascending colon. The mRNA analysis revealed no changes associated with EcN feeding. In conclusion, according to our analyses EcN has only minor effects on the distribution of mucosal immune cells in the gut of healthy individuals. The well-established preventive effects of EcN might therefore be relate to other mechanisms than simple modulation of immune cell distribution.
PMID: 16530848 [PubMed - indexed for MEDLINE]
1: Arch Anim Nutr. 2005 Dec;59(6):419-27. Links
Effects of feeding antibiotic-free creep feed supplemented with oligofructose, probiotics or synbiotics to suckling piglets increases the preweaning weight gain and composition of intestinal microbiota.
Shim SB, Verstegen MW, Kim IH, Kwon OS, Verdonk JM.
Animal Nutrition Group, Department of Animal Science, Wageningen University, Wageningen, The Netherlands. soobo.shim@wur.nl
The objective of this study was to determine whether feeding an antibiotic-free creep feed supplemented with either oligofructose, probiotics or synbiotics to suckling piglets influences growth performance, the gut microflora, gut morphology and hematological traits at weaning. Twenty sows with 10 piglets each were randomly assigned to one of four treatments. The treatments consisted of a control (antibiotic-free) diet, 0.2% oligofructose (OF), 0.3% probiotics or 0.5% synbiotics (mixture of 0.2% OF+0.3% probiotics). Piglets were offered the diet ad libitum from 7 d after birth until one day after weaning (21 d of age). At the day after weaning, blood samples were collected from the jugular vein to determine the immune response. Digesta samples of the ileum and colon were collected to determine the microbial composition. Tissue segments from the duodenum and ileum were collected for morphometric measurements of the small intestine. The average daily weight gain was significantly higher for piglets fed the OF or synbiotics diet compared with the pigs fed the control diet. The hematological traits (the concentration of lymphocytes and neutrophils in whole blood) were not affected by the diet. Piglets fed the OF, probiotics or synbiotics diet had a significantly decreased number of total coliform bacteria in the colon. Feeding OF, probiotics or synbiotics significantly increased the population of bifidobacteria in the ileum compared to the control. In the colon, the probiotics and synbiotics diet significantly increased the number of bifidobacteria compared with the control diet. The results of this experiment showed that supplementation of oligofructose or synbiotics to an antibiotic-free creep feed during the preweaning period affected gut microbial population and performance of piglets.
PMID: 16429827 [PubMed - indexed for MEDLINE]
1: Foodborne Pathog Dis. 2005 Winter;2(4):317-29. Links
Effect of porcine-derived mucosal competitive exclusion culture on antimicrobial resistance in Escherichia coli from growing piglets.
Kim LM, Gray JT, Bailey JS, Jones RD, Fedorka-Cray PJ.
Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA, ARS, Athens, Georgia 30605, USA.
While use of antimicrobial drugs in livestock production has made a significant impact on animal health, welfare, and productivity, interest in suitable alternatives such as pre/probiotics, organic acids, and cultures of normal flora or "competitive exclusion" cultures from young animals has increased significantly in the wake of the antimicrobial resistance issue. The present study was undertaken to determine the effect of porcine-derived mucosal competitive exclusion (PCE) culture on both the antimicrobial susceptibility of commensal E. coli and on growth performance in piglets. Two replicate trials were conducted using growing piglets fed standard antimicrobial-free production diets. Piglets in the treatment group were orally dosed with PCE (10(10) cfu/mL) twice within 24 h of birth, at weaning, and 18-24 h post-weaning; control group piglets were dosed with sterile broth as a placebo. Fecal samples from all piglets were cultured for commensal E. coli at dosing times and when feed type was changed. A significantly higher proportion of E. coli from PCE-treated piglets demonstrated resistance to tetracycline (p < 0.0001), and streptomycin (p < 0.0001) when compared to controls. Resistance to streptomycin resistance in E. coli from piglets treated with PCE culture was variable, returning to baseline levels by day 21 (weaning). Piglets treated with the PCE culture demonstrated improved feed efficiencies when compared to control piglets (p < 0.005) during feeding of the starter and first growth diets. The PCE culture used in the present study had previously been shown to effectively exclude Salmonella in pigs. To the best of the authors' knowledge, this is the first report characterizing the effect of a competitive exclusion culture on antimicrobial resistance of commensal E. coli.
PMID: 16366854 [PubMed - indexed for MEDLINE]
1: J Anim Sci. 2007 Dec 11 [Epub ahead of print] Links
Use of phytogenic products as feed additives for swine and poultry.
Windisch WM, Schedle K, Plitzner C, Kroismayr A.
Department of Food Science and Technology, Division of Animal Food and Nutrition, University of Natural Resources and Applied Life Sciences, Vienna, 1180 Vienna, Austria.
This article summarizes experimental knowledge on efficacy, possible modes of action, and aspects of application of phytogenic products as feed additives for swine and poultry. Phytogenic feed additives comprise a wide variety of herbs, spices, and products derived thereof, and are mainly essential oils. The assumption that phytogenic compounds might improve palatability of feed has not been confirmed yet by choice feeding studies. Although numerous studies have been demonstrating antioxidative and antimicrobial efficacy in vitro, respective experimental in vivo evidence is still quite limited. The same applies to the supposition that phytogenic compounds may specifically enhance activities of digestive enzymes and nutrient absorption. Nevertheless, a limited number of experimental comparisons of phytogenic feed additives with antibiotics and organic acids suggested similar effects on the gut, such as reduced bacterial colony counts, less fermentation products (including ammonia and biogenic amines), less activity of the gut-associated lymphatic system, and a greater pre-cecal nutrient digestion, probably reflecting an overall improved gut equilibrium. In addition, some phytogenic compounds seem to promote intestinal mucus production. Such effects may explain a considerable number of practical studies with swine and poultry reporting improved production performance after providing phytogenic feed additives. In total, available evidence indicates that phytogenic feed additives may add to the set of non-antibiotic growth promoters, such as organic acids and probiotics, for use in livestock. However, a systematic approach on the efficacy and safety of phytogenic compounds used as feed additives for swine and poultry is still missing.
PMID: 18073277 [PubMed - as supplied by publisher]
1: Proc Nutr Soc. 2003 Feb;62(1):73-80. Links
Influences of probiotic bacteria on organic acid production by pig caecal bacteria in vitro.
Sakata T, Kojima T, Fujieda M, Takahashi M, Michibata T.
Department of Basic Sciences, Ishinomaki Senshu University, 986-8580 Ishinomaki, Japan. sakata@isenshu-u.ac.jp
The mechanism of action of probiotics is largely unknown. A potential mechanism should be to increase the production of short-chain fatty acids (SCFA), known modulators of gut functions, by the bacterial ecosystem in the large intestine. The present paper reviews our recent studies in which the capacity of probiotic bacteria to increase the production of SCFA by pig caecal bacteria was investigated using batch-culture and continuous-culture techniques. All four commercial probiotic preparations and three strains of probiotic bacteria dose-dependently accelerated the net production of SCFA, succinic acid and lactic acid without changing the acid profile, and slowed the net production of NH4. Effects on organic acid production did not vary among different probiotic species. Neither probiotic preparations nor probiotic bacteria affected the organic acid production from glucose, gastric mucin, starch or lactose, or organic acids produced:added saccharide. Glucose abolished these effects of probiotic preparations. However, the capacity of probiotics to increase SCFA production was not modified by gastric mucin, starch or lactose. These results indicate that probiotic bacteria increase SCFA production by accelerating the breakdown of carbohydrates that are resistant to indigenous bacteria, and suggest that the concept of prebiotics in terms of SCFA production as a measure of probiotic function is arguable.
1: Proc Nutr Soc. 2007 May;66(2):260-8. Links
Nutritional management of gut health in pigs around weaning.
Lallès JP, Bosi P, Smidt H, Stokes CR.
INRA, UMR1079, Rearing Systems, Animal and Human Nutrition, F-35590 Saint-Gilles, France. Jean-Paul.Lalles@rennes.inra.fr
Early weaning of piglets is often accompanied by a severe growth check and diarrhoea. It is well established that this process is multi-factorial and that post-weaning anorexia and undernutrition are major aetiological factors. Gastrointestinal disturbances include alterations in small intestine architecture and enzyme activities. Recent data indicate transiently-increased mucosal permeability, disturbed absorptive-secretory electrolyte balance and altered local inflammatory cytokine patterns after weaning. These responses appear to operate according to two distinct temporal patterns, an acute response followed by a long-lasting adaptation response. Pigs coexist with a diverse and dense commensal microbiota in their gastrointestinal tract. Most of these microbes are beneficial, providing necessary nutrients or protection against harmful pathogens for the host. The microbial colonisation of the porcine intestine begins at birth and follows a rapid succession during the neonatal and weaning period. Following the withdrawal of sow's milk the young piglets are highly susceptible to enteric diseases partly as a result of the altered balance between developing beneficial microbiota and the establishment of intestinal bacterial pathogens. The intestinal immune system of the newborn piglet is poorly developed at birth and undergoes a rapid period of expansion and specialisation that is not achieved before early (commercial) weaning. Here, new insights on the interactions between feed components, the commensal microbiota and the physiology and immunology of the host gastrointestinal tract are highlighted, and some novel dietary strategies are outlined that are focused on improving gut health. Prebiotics and probiotics are clear nutritional options, while convincing evidence is still lacking for other bioactive substances of vegetable origin.
PMID: 17466106 [PubMed - indexed for MEDLINE]
1: J Appl Microbiol. 1998 Jan;84(1):68-71. Links
Effect of probiotic CenBiot on the control of diarrhoea and feed efficiency in pigs.
Zani JL, da Cruz FW, dos Santos AF, Gil-Turnes C.
Centro de Biotecnologia, Universidade Federal de Pelotas, Campus Universitario, Pelotas, RS, Brazil.
The production and testing of a probiotic formulated with a strain of Bacillus cereus (Probiotic CenBiot) is reported. The strain was grown in fed batch fermenters, desiccated, and mixed with cornflour. To test the effect of the probiotic in controlling piglet diarrhoea, two groups of sows and their respective litters received feed supplemented with probiotic CenBiot or Furazolidone; the control group received the same basic feed but not supplemented. Probiotic CenBiot was as effective as Furazolidone, reducing the prevalence of diarrhoea to half that in the control group. It also significantly improved feed conversion, daily weight gain and total weight gain in pigs in the nursery phase. Feed conversion ratios of weaned pigs were 1.904, 2.146 and 2.099 for the groups that received Probiotic CenBiot, a commercial probiotic, and basic feed, respectively. In terms of food consumption, the Probiotic CenBiot group consumed 12.6% less than the commercial probiotic group, and 10% less than the control group, to achieve the same weight.
PMID: 15244059 [PubMed - indexed for MEDLINE] |
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