碱式氯化铜在水产动物中的应用研究

wangshangchu · 发表于 2013-11-19 10:52:07

碱式氯化铜相对硫酸铜更稳定，利用率更高，对环境排放污染少等诸多优势，已经被行业普遍接受。现阶段碱式氯化铜在畜禽动物饲料中应用已经十分普遍，但是在水产动物中的应用研究相对十分缺乏。而这是一篇关于碱式氯化铜在水产动物中的应用研究文章，并提出了合理添加使用建议。个人觉得很有参考价值，并且今后有必要对其他的水产动物进行碱式氯化铜的应用研究。

Effects of tribasic copper chloride on growth, copper status, antioxidant activities, immune responses and intestinal microflora of blunt snout bream (Megalobrama amblycephala) fed practical diets

Xian-Ping Shaoa, b, Wen-Bin Liua, Kang-Le Lua, Wei-Na Xua, Wei-Wei Zhanga, Ying Wanga, b, Jie Zhua

a Key Laboratory of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China

b Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China

Aquaculture

Volumes 338–341, 29 March 2012, Pages 154–159

Abstract

A study was conducted to investigate the effects of dietary tribasic copper chloride on growth, copper status, antioxidant activities, immune responses and intestinal microflora in blunt snout bream, Megalobrama amblycephala. Eight practical diets containing different levels of copper (0, 3, 6, 9, 25, 50, 100 and 150 mg Cu kg− 1) from tribasic copper chloride were fed to blunt snout bream (31 g of initial average body weight) in quadruplicate groups in outdoor cages. Growth, copper status, antioxidant activities, immune responses and intestinal microflora were analyzed after a 56-day feeding period. Weight gain and specific growth rates in fish fed diets supplemented with 3–6 mg Cu kg− 1 were significantly higher than those in fish fed the control diet (which supplemented with 0 mg Cu kg− 1) (p < 0.05). Fish fed diets supplemented with 9 mg Cu kg− 1 had significantly lower feed conversion ratio than fish fed the control diets (p < 0.05). Copper concentrations in liver, kidney and intestine were significantly higher in fish fed diets with 50–150 mg Cu kg− 1 than those in fish fed the control diet (p < 0.05). Dietary copper at levels ranging from 9 mg kg− 1 to 100 mg kg− 1 significantly enhanced T-AOC activities (p < 0.05). In addition, diets supplemented with 100–150 mg Cu kg− 1 significantly increased activities of AKP and ACP (p < 0.05).Intestinal bacterial community structures in fish fed diets supplemented with 6–150 mg Cu kg− 1 were different from those in fish fed diets supplemented with 0–3 mg Cu kg− 1. The study showed that diets supplemented with 3–6 mg Cu kg− 1 could meet the copper requirements for blunt snout bream fed practical diets; diets supplemented with 9 mg Cu kg− 1 could increase feed efficiency; diets supplemented with 100 mg Cu kg− 1 could increase activities of AKP and ACP and affect intestinal microflora structure, though leading to a status of increasing copper accumulation in tissues.

Keywords

Blunt snout bream (Megalobrama amblycephala); Tribasic copper chloride; Growth; Antioxidant activity;Immune response; Intestinal microflora

碱式氯化铜对饲喂基础日粮的团头鲂生长、铜状况、抗氧化活性、免疫应答及肠道菌群的影响

邵先平，南京农业大学

摘要：

本试验旨在研究日粮中碱式氯化铜对团头鲂生长、铜状况、抗氧化活性、免疫应答和肠道菌群的影响。8组实用日粮，分别添加了不同水平的碱式氯化铜(0, 3, 6, 9, 25, 50, 100 and 150 mg Cu kg−1) 来饲喂（初始重31g）团头鲂，每组四个重复，露天桶养。饲喂56天后，对团头鲂生长、铜状况、抗氧化活性、免疫应答和肠道菌群进行分析。3-6mg Cu kg− 1 日粮组团头鲂增重和特定生长率显著高于对照组（添加0 mg Cu kg− 1） (p < 0.05)。9 mg Cu kg− 1 日粮组团头鲂饵料系数显著低于对照组(p < 0.05)。饲喂50–150 mg Cu kg− 1日粮组团头鲂铜在肝脏、肾脏和肠道蓄积显著高于对照组(p < 0.05)。铜添加水平从9 mg kg− 1 到 100 mg kg− 1 ，均显著提高T-AOC活力(p < 0.05)。此外，添加100–150 mg Cu kg− 1日粮组显著提高了AKP和ACP活力(p < 0.05)。6–150 mg Cu kg− 1 日粮组团头鲂肠道菌群结构与0–3 mg Cu kg− 1日粮组有差异。研究显示，在日粮中添加3–6 mg Cu kg− 1 可以满足团头鲂对实用日粮铜的需求；在日粮中添加9 mg Cu kg− 1 能提高饲料效率；在日粮中添加100 mg Cu kg− 1虽然增加了组织的铜蓄积，但是还可以提高AKP和ACP活力，影响肠道菌群结构。

关键词：团头鲂；碱式氯化铜；生长；抗氧化活性；免疫应答；肠道菌群