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现在的蛋鸡、蛋种鸡肿瘤病多么?

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发表于 2008-1-24 17:44:27 | 显示全部楼层 |阅读模式
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Avian leukosis virus (ALV) subgroup J (ALV-J) is an exogenous ALV and causes myeloid leukosis in meat-type chickens. We have previously reported the isolation and identification of ALV-J in commercial layer flocks from 12 farms in northern China. In this report, we further characterized this virus by in situ polymerase chain reaction (PCR) hybridization in various affected organs of chickens from six of the 12 farms. A routine method for hybridization of nucleic acid uses radioactive probe, such as a P(32)-labelled probe. We found that the non-radioactive digoxigenin (DIG) probe is sensitive enough to detect the nucleic acid of virus in chicken tissues. We used a pair of published primers (H5/H7) specific to the gp85 envelope gene and 3' region of pol gene of prototype ALV-J strain HPRS-103. The total RNA extracted from tumour, bone marrow, oviduct, liver and spleen of the diseased chickens from six commercial flocks, and cDNA was successfully amplified. Using the primers and cDNA, we obtained an ALV-J-specific cDNA probe of 545 bp in length by PCR. In situ PCR with H5/H7 primers was carried out in the paraffin sections from tissues of the diseased chickens, followed by in situ hybridization using the DIG-labelled cDNA probe. Positive hybridization signals were detected in the cytoplasm of paraffin sections of tumours and other organ tissues. The intensity of the signals was documented using an image analysis system measuring integral optical density (IOD). The IOD values for tissue sections treated by in situ PCR hybridization are significantly higher than that by in situ hybridization alone (P < 0.01). These data taken together suggest that in situ PCR hybridization is a more sensitive technique for detection of ALV-J in tissue sections.
目前饲养在我国北方的蛋鸡、蛋种鸡是否肿瘤多见?如果有肿瘤,是否肾脏肿瘤多见?另外,法氏囊、骨骼上是否有油脂样增生物?
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发表于 2008-8-15 21:24:39 | 显示全部楼层
现比较多见。
发表于 2008-8-16 06:44:42 | 显示全部楼层
现在有治疗的方法吗
发表于 2008-8-22 16:47:29 | 显示全部楼层
肿瘤多为病毒病,很难治疗的。
发表于 2008-8-24 14:41:43 | 显示全部楼层
肿瘤病对于鸡来说就是癌症,是不能治疗的,只有通过源头的净化,应用阴性鸡去传代生产。
不过有时候采用一些大蒜之类的药物能否起到一点作用。
发表于 2008-9-3 01:18:00 | 显示全部楼层
一直很多的,记得2000年解剖课共解剖了17只淘汰蛋鸡,基本都有不止一个肿瘤,平均3个!搞得好几天不吃鸡肉
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